{"product_id":"9781071606186","title":"CRISPR-Cas Methods","description":"\u003cdiv\u003eThis volume details the fundamentals of the \u003ci\u003eCRISPR-Cas system\u003c\/i\u003e, and its protocols illustrate advances in \u003ci\u003eCRISPR-Cas\u003c\/i\u003e techniques for efficient genome editing. Introductory chapters provide a wide horizon of \u003ci\u003eCRISPR\/Cas-based methods\u003c\/i\u003e and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. \u003c\/div\u003e\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\u003cdiv\u003eAuthoritative and cutting-edge, \u003ci\u003eCRISPR-Cas Methods \u003c\/i\u003eaims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.\u003c\/div\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePublication Year: \u003c\/strong\u003e2020\u003cbr\u003e\u003cstrong\u003eImprint: \u003c\/strong\u003eSpringer US\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"M. Tofazzal Islam","offers":[{"title":"Default Title","offer_id":41264190914738,"sku":"9781071606186","price":179.39,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0502\/5382\/4178\/products\/1071606182.01_SCLZZZZZZZ.jpg?v=1635992071","url":"https:\/\/readabook.store\/products\/9781071606186","provider":"READABOOK BY ALKEM","version":"1.0","type":"link"}