{"product_id":"9781493980987","title":"Synthetic mRNA","description":"This volume\npresents detailed laboratory protocols for in\nvitro synthesis of mRNA with favorable properties, its introduction into\ncells by a variety of techniques, and the measurement of physiological and\nclinical consequences such as protein replacement and cancer immunotherapy. Synthetic\ntechniques are described for structural features in mRNA that provide investigational\ntools such as fluorescence emission, click chemistry, photo-chemical\ncrosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced\nactivation of the innate immune response. Protocols are described for clinical\napplications such as large-scale transfection of dendritic cells, production of\nGMP-grade mRNA, redirecting T cell specificity, and use of molecular\nadjuvants for RNA vaccines. Written\nin the highly successful Methods in\nMolecular Biology series format, chapters include introductions to their\nrespective topics, lists of the necessary materials and reagents, step-by-step\nlaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\n\nSynthetic\nmRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resource\nfor both laboratory investigators and clinicians interested in this powerful\nand rapidly evolving technology. \u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePublication Year: \u003c\/strong\u003e2016\u003cbr\u003e\u003cstrong\u003eImprint: \u003c\/strong\u003eSpringer New York\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003eFormat: P\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003eWeight (Gram): 633\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Robert E. Rhoads","offers":[{"title":"Default Title","offer_id":41246503207090,"sku":"9781493980987","price":155.99,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0502\/5382\/4178\/products\/149398098X.01_SCLZZZZZZZ.jpg?v=1635649121","url":"https:\/\/readabook.store\/products\/9781493980987","provider":"READABOOK BY ALKEM","version":"1.0","type":"link"}